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Registros recuperados: 76
Primeira ... 1234 ... Última
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Lipid nutrition and settlement behaviour in American lobster Homarus americanus ArchiMer
Theriault, I; Pernet, Fabrice.
This study examines the effects of varying dietary lipids on growth, survival, lipid composition and behaviour in lobster Homarus americanus (Milne Edwards, 1837) postlarvae. Lobsters were fed one of 3 diets during their early ontogeny: (1) Artemac, a widely used Artemia replacement; (2) a ternary mixture consisting of Artemac, frozen Artemia adults and Artemia flakes; or (3) a mixture of live A. salina nauplii and adults. We evaluated the olfactory capacity (response to predator odour plume) and cryptic behaviour (ability to hide during settlement) of lobster postlarvae as a function of these diets and the lipid and the fatty acid composition of lobsters. Growth and survival of lobster postlarvae were highest in those fed live Artemia, followed by the...
Tipo: Text Palavras-chave: Settlement behaviour; Nutrition; Lipid; Habitat selection; Fatty acid; Crustacean larvae.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-4651.pdf
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Effect of Rhodomonas salina addition to a standard hatchery diet during the early ontogeny of the scallop Pecten maximus ArchiMer
Tremblay, Rejean; Cartier, Simon; Miner, Philippe; Pernet, Fabrice; Quere, Claudie; Moal, Jeanne; Muzellec, Marie-louise; Mazuret, Michel; Samain, Jean-francois.
The main objective of this study was to identify algal diets that maximize the survival and growth and alter the biochemical content of Pecten maximus larvae with the aim of improving metamorphosis. We also evaluated the potential of the cryptophyceae Rhodomonas salina as a food source for these larvae. Two flagellates, Isochrysis aff. galbana (T) and Pavlova lutheri (P), and two diatoms, Chaetoceros gracilis (C) or Skeletonema costatum (S), were tested as two ternary diets, namely PTC and PTS. PTC and PTS were compared with diets that also included R. salina (R). The addition of R. salina and the replacement of C. gracilis by S. costatum in the traditional hatchery diet seem to be two interesting alternatives for increasing the productivity of larval...
Tipo: Text Palavras-chave: Algal fatty acids and sterols; Energetic content; Metamorphosis; Nutrition; Scallop.
Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2404.pdf
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Remodeling of membrane lipids in gills of adult hard clam Mercenaria mercenaria during declining temperature ArchiMer
Parent, G; Pernet, Fabrice; Tremblay, R; Sevigny, J; Ouellette, M.
In a previous study, a major remodeling of lipids, consistent with the theory of homeoviscous adaptation, was observed in the juvenile hard clam Mercenaria mercenaria during a temperature reduction from 24 to -1 degrees C. In addition, the lipid remodeling varied between genetically distinct lines of hard clams. The present study examined whether adult hard clams originating from different locations and a selectively bred variety show differences related to their genetic characteristics in the remodeling of lipids that normally occur during decreasing temperatures. Wild hard clams from 4 locations in Atlantic Canada and the selectively bred M mercenaria var. notata were held at an aquaculture growout site located at the northern distribution limit of the...
Tipo: Text Palavras-chave: Sterol; Phospholipid; Membrane lipid; Intraspecific variation; Food; Fatty acid; Bivalve; Allozymes; Acclimatization.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-4743.pdf
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Lipid requirements of the scallop Pecten maximus (L.) during larval and post-larval development in relation to addition of Rhodomonas sauna in diet ArchiMer
Gagne, Renee; Tremblay, Rejean; Pernet, Fabrice; Miner, Philippe; Samain, Jean-francois; Olivier, Frederic.
The main objective of this study was to evaluate the effect of the addition of Rhodomonas salina in the diet of Pecten maximus on growth, survival and metamorphosis success in relation to biochemical content. Food quality is an essential factor in the success of larval, post-larval and juvenile development. A diet rich in polyunsaturated fatty acids present an advantage for growth, survival and metamorphosis success. Larvae and post-larvae fed with the diet containing R. sauna showed a higher accumulation of arachidonic acid (20:4n-6) and generally a lower concentration of docosahexaenoic acid (22:6n-3). Addition of R. sauna in diet was related with higher level of eicosapentaenoic acid (20:5n-3) in larval stages, but lower level in post-larvae. Addition...
Tipo: Text Palavras-chave: Nutrition; Biochemical characteristics; Fatty acids; Sterol profiles.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00019/13045/10205.pdf
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Environmental anomalies, energetic reserves and fatty acid modifications in oysters coincide with an exceptional mortality event ArchiMer
Pernet, Fabrice; Barret, Jean; Marty, Claire; Moal, Jeanne; Le Gall, Patrik; Boudry, Pierre.
Mortality of oysters Crassostrea gigas occurred along the coasts of France during summer 2008, causing a serious crisis in French oyster production. During spring to summer 2008, 5 groups of 1-yr old oysters of different origins and ploidy levels deployed in the Thau lagoon (France) were sampled for biometrical and biochemical analyses; environmental parameters were monitored in the same area. Mortality occurred in 2 episodes: 13 May to 10 June and 24 June to 9 July. Wild-collected oysters showed mortality sooner than other groups but total overall mortality was 85% in all groups. Mortality occurred when seawater temperature was >19 degrees C during the reproductive season when energetic reserves of oysters were at their lowest. Energy reserve levels...
Tipo: Text Palavras-chave: Bivalves; Energetic reserve; Environment; Fatty acid; Summer mortality; Trophic signatures; Temperature.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00003/11388/7964.pdf
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Mortalité du naissain d’Huître creuse Crassostrea gigas dans l’étang de Thau en 2009 ArchiMer
Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Malet, Nathalie; Pastoureaud, Annie; Munaron, Dominique; De Lorgeril, Julien; Bachere, Evelyne; Vaquer, Andre; Huvet, Arnaud; Corporeau, Charlotte; Normand, Julien; Boudry, Pierre; Moal, Jeanne; Quere, Claudie; Quilien, Virgile; Daniel, Jean-yves; Pepin, Jean-francois; Saulnier, Denis; Gonzalez, Jean-louis.
This study was conducted in the Mediterranean area during a mass mortality event of Pacific oyster Crassostrea gigas. The objectives of this work were to increase our understanding of the mass mortality phenomena in Pacific oysters, to identify risky cultivation practises and to propose mitigation strategies. In the first part of this work, we followed environmental parameters of the Thau lagoon in relation with mortality events. Then, we compared mortality and growth parameters of oysters as a function of their origins, their development stages and cultivation sites in relation with energetic, reproduction and pathogens. Finally, we conducted transfer experiments where oysters maintained free of mortality in open sea were transferred in the Thau lagoon...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00002/11354/7927.pdf
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Temperature effect on survival, growth, and triacylglycerol content during the early ontogeny of Mytilus edulis and M. trossulus ArchiMer
Rayssac, Nathalie; Pernet, Fabrice; Lacasse, Olivia; Tremblay, Rejean.
Temperature is a major factor contributing to the biogeography of intertidal poikilotherms. The population dynamics of intertidal invertebrates like mussels are strongly dependent on larval supply and larval mortality. Unlike adults, which are resistant to fluctuating environmental conditions, larvae are highly sensitive to the physicochemical conditions of the pelagic zone. The effects of rearing temperature on larval and post-larval performance of 2 coexisting mussels species, Mytilus edulis and M. trossulus, were examined in this study. In the first experiment, larvae of both species were reared separately at constant temperatures of 10, 17, and 24 degrees C from D-larvae to the dissoconch stage. In the second experiment, pediveligers were reared under...
Tipo: Text Palavras-chave: Thermal adaptation; Bivalve mollusk; Selection; Biogeography; Lipid; Larval ecology.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00019/13028/10159.pdf
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The importance of turbulent kinetic energy on transport of juvenile clams (Mya arenaria) ArchiMer
Redjah, I.; Olivier, Frederic; Tremblay, R.; Myrand, B.; Pernet, Fabrice; Neumeier, U.; Chevarie, L..
Soft-shell clam, Mya arenaria, culture on the east coast of Canada is characterized by high loss following seeding. To evaluate the importance of passive transport due to currents, an experimental flume study was designed. The purpose was to measure the effects of hydrodynamic conditions, substrate, and clam size on dispersal in controlled laboratory condition and to interpret these results in relation to field measurements. Unidirectional currents with gradual increasing velocities (0 to 60 cm s(-1)) were applied to three substrates (muddy sand, medium sand, and coarse sand) in which clams from one of three size classes (10, 15, and 20 mm) had burrowed. We also examined the resulting effects of turbulent kinetic energy on the erosion of medium-grain sand...
Tipo: Text Palavras-chave: Clam transport; Substratum erosion; Unidirectional currents; Turbulence; ADV; Flume.
Ano: 2010 URL: http://archimer.ifremer.fr/doc/00014/12507/9368.pdf
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Low settlement of the pacific oysters Crassostrea gigas in the Thau lagoon reflects competition for space and food ArchiMer
Rayssac, Nathalie; Malet, Nathalie; Pernet, Fabrice.
The objective of this work is to understand why oysters Crassostrea gigas shows low settlement success in the Thau lagoon despite the fact that biomass of adults is elevated and that larvae show normal development in the water column. Our hypotheses are that oysters compete for food or space with other filter-feeders animals such as sponges, ascidians and other bivalves. To test the trophic hypothesis, we analysed the entire settler community harvested on four-month-old collectors deployed in the Thau lagoon during the summer 2009 for stable isotopes and fatty acids. Based on the isotopic, crustacean amphipods and barnacles can be rejected as a potential food competitor of juvenile oysters. However, other bivalves such as Mytilus sp., Anomia sp., and...
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Ano: 2010 URL: http://archimer.ifremer.fr/doc/00015/12665/9555.pdf
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Suivi national des mortalités au stade naissain d'un lot témoin et d'un lot à survie améliorée pour Crassostrea gigas ArchiMer
Degremont, Lionel; Maurouard, Elise; Nourry, Max; Seugnet, Jean-luc; Bedier, Edouard; Fleury, Elodie; Langlade, Aime; Pernet, Fabrice; Pouvreau, Stephane; Le Souchu, Pierrick; Normand, Julien; D'Amico, Florence; Rumebe, Myriam; Cantin, Christian; Barret, Jean; Le Gall, Patrik; Baud, Jean-pierre; Cochennec-laureau, Nathalie; Gervasoni, Erika; Pelissier, Pablo.
Ce rapport présente la comparaison des mortalités de deux lots de naissains de Crassostrea gigas dans plusieurs bassins conchylicoles et déterminer le gain de survie obtenu par la sélection pour des huîtres diploïdes
Tipo: Text Palavras-chave: Mortalités; Huître creuse; Crassostrea gigas; Sélection génétique; Naissain.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00065/17644/15180.pdf
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Observatoire National Conchylicole. Campagne 2011 ArchiMer
Fleury, Elodie; D'Amico, Florence; Annezo, Jean-pierre; Barret, Jean; Blin, Jean Louis; Bouget, Jean-francois; Claude, Serge; Guilpain, Patrice; Grizon, James; Hitier, Benoist; Langlade, Aime; Le Gall, Patrik; Le Souchu, Pierrick; Martin, Anne-genevieve; Mary, Charlotte; Normand, Julien; Parrad, Sophie; Penot, Julia; Pernet, Fabrice; Pien, Sebastien.
Depuis 2009, l'Observatoire Conchylicole assure le suivi, sur des sites ateliers disposés sur l'ensemble du littoral français, de lots sentinelles d'huîtres creuses Crassostrea gigas représentatifs de la production française. Leur suivi permet d'acquérir des données nationales de croissance et de mortalité, de traduire la dynamique spatio-temporelle des performances d’élevage et ainsi de participer à la compréhension des phénomènes observés. Pour ce faire, des lots sentinelles d’huîtres correspondant à différentes origines (captage ou écloserie, diploïdes ou triploïdes) et à différents stades d’élevage (naissain ou adultes 18 mois) sont déployés simultanément sur 13 sites ateliers représentant les grandes régions conchylicoles du littoral français. En...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00083/19433/17047.pdf
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Les surmortalités des naissains d’huîtres creuses, Crassostrea gigas : acquis des recherches en 2010 ArchiMer
Cochennec-laureau, Nathalie; Baud, Jean-pierre; Pepin, Jean-francois; Benabdelmouna, Abdellah; Soletchnik, Patrick; Lupo, Coralie; Garcia, Celine; Arzul, Isabelle; Boudry, Pierre; Huvet, Arnaud; Pernet, Fabrice; Bachere, Evelyne; Bedier, Edouard; Petton, Bruno; Gaussem, Florian; Stanisiere, Jean-yves; Degremont, Lionel.
Depuis 2008, la filière ostréicole française doit faire face à des surmortalités exceptionnelles des naissains d’huîtres creuses, Crassostrea gigas, (huîtres de moins d’un an) comprises entre 60 et 90% dans tous les sites d’élevage. Des cas de mortalité similaires ont été décrits en Irlande, dans les îles anglo-normandes, au Portugal en 2009 et au Royaume-Uni en 2010. Récemment deux épisodes de mortalité, associés à la présence d’un virus OsHV1 μvar (ou un variant très proche) ont été également décrits en Nouvelle Zélande (décembre 2010) et en Australie (janvier 2011) soulignant la vulnérabilité de la filière ostréicole dans le monde. L’objectif de ce document est de faire un point sur les recherches menées depuis l’apparition de ce phénomène et de...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00033/14423/11719.pdf
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Responses of diploid and triploid Pacific oysters Crassostrea gigas to Vibrio infection in relation to their reproductive status ArchiMer
De Decker, Sophie; Normand, Julien; Saulnier, Denis; Pernet, Fabrice; Castagnet, Sophie; Boudry, Pierre.
Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio...
Tipo: Text Palavras-chave: Experimental vibriosis; Crassostrea gigas; Vibrio; Survival distribution function; Triploidy; Reproductive effort.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00030/14108/11368.pdf
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Physiological and biochemical indicators of mussel seed quality in relation to temperatures ArchiMer
Tremblay, Rejean; Landry, Thomas; Leblanc, Neil; Pernet, Fabrice; Barkhouse, Carla; Sevigny, Jean-marie.
The bivalve's aquaculture industry is an important component of the economy in Eastern Canada. Seed collection is an initial and critical activity in most bivalve aquaculture industries including mussel farming in Prince Edward Island, production is entirely dependent on natural spat collection. Although seed supply is not a concern from a quantitative standpoint, there are growing concerns about the quality of natural seed. The general objective of this study was to identify and assess mussel seed quality criteria on the basis of physiological and biochemical status under laboratory and field conditions. The performance, as estimated by metabolic measurements, lipid class composition, multi-locus heterozygosity (MLH) and survival to stressful environment...
Tipo: Text Palavras-chave: Wild seed; Aquaculture; Physiological fitness; Growth; Survival; Multi-locus heterozygosity; Mytilus spp..
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00051/16232/13754.pdf
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Mortalités massives de l’Huître creuse: causes et perspectives ArchiMer
Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Lagarde, Franck; Fiandrino, Annie; Huvet, Arnaud; Corporeau, Charlotte; Boudry, Pierre; Quere, Claudie; Degremont, Lionel; Pepin, Jean-francois; Saulnier, Denis; Boulet, Hélène; Keck, Nicolas.
The objectives of this study are to increase our understanding of the mass mortality phenomena in Pacific oysters Crassostrea gigas in the Thau lagoon and to examine the effect of cultivation practises. Mortality events in 2009 and 2010 always coincide with simple or dual infections involving primarily the Herpes virus OsHV-I and secondarily the bacteria Vibrio splendidus, when seawater temperature is between 17°C and 24°C. In the Thau lagoon, mortality starts early May and last until fall with an interruption period when seawater temperature is higher than 24°C in July and August. Mortality of oysters varies as a function of zootechnical life-history, hydrodynamics and cultivation practises. First, mortality depends on whether or not oysters have been...
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Ano: 2011 URL: http://archimer.ifremer.fr/doc/00043/15404/12764.pdf
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Bacterial colonization of winter flounder Pseudopleuronectes americanus fed live feed enriched with three different commercial diets ArchiMer
Seychelles, L. H.; Audet, C.; Tremblay, R.; Lemarchand, K.; Pernet, Fabrice.
The proliferation of bacteria in intensive aquaculture systems may be responsible for poor growth and mass mortality of marine fish larvae. Essential fatty acids provided in the diet could protect larvae by modulation of the immune response via arachidonic acid (AA) and eicosapentaenoic acid (EPA). Winter flounder Pseudopleuronectes americanus larvae were fed rotifers Brachionus plicatilis enriched with three commercial diets containing different fatty acid profiles. Bacterial colonization on the gills and skin and in the intestinal lumen was evaluated at the end of the rotifer feeding period (day 26), and growth was surveyed until metamorphosis. At 26 days post hatching, larvae fed rotifers containing the higher AA content and with a higher...
Tipo: Text Palavras-chave: Bacteria; Colonization; Fatty acids; Live feed; Winter flounder.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00031/14273/12399.pdf
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Physiological and biochemical changes associated with massive mortality events occurring in larvae of American oyster (Crassostrea virginica) ArchiMer
Genard, Bertrand; Pernet, Fabrice; Lemarchand, Karine; Boudry, Pierre; Moraga, Dario; Tremblay, Rejean.
In this paper, biochemical and physiological analyses were used to characterize changes associated with mortality event occurred during veliger development of American oyster, Crassostrea virginica. Biochemical analyses included the evaluation of lipid classes, fatty acid composition and total protein content. Larval physiology was evaluated by studying feeding activity, enzymes related to energy metabolism, oxidative stress levels and enzymatic antioxidant defenses. These analyses were complemented by bacterial community analyses as well as by measuring larval oyster performance. We observed that mortality events coincided with (1) strong changes in the surrounding bacterial community; (2) a progressive decrease in feeding activity; (3) higher levels of...
Tipo: Text Palavras-chave: Massive mortality; Larvae; Microbial environment; Lipid; Fatty acids; Energy metabolism; Oxidative stress; Antioxidant enzymes; Crassostrea virginica.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00051/16231/13753.pdf
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Les pratiques culturales peuvent-elles permettre de réduire la surmortalité du naissain d'huîtres creuses? Récapitulatif des essais d'élevage et expérimentations zootechniques menés sur le territoire français entre 2008 et 2010 ArchiMer
Soletchnik, Patrick; Mazurie, Joseph; Allain, Gwenhael; Bedier, Edouard; Benabdelmouna, Abdellah; Blin, Jean-louis; Bouquet, Anne-lise; Cochet, Hélène; Degremont, Lionel; Gaussem, Florian; Gervasoni, Erika; Glize, Philippe; Petton, Bruno; Roussel, Pierre-yves; Pernet, Fabrice.
This paper summarizes knowledge about relations between husbandry practices and oyster spat mortalities since 2008 in France. These relations are strictly analyzed from a panel of experiments set on the shore (in outdoor experiments) by Ifremer teams and technical institutes all over French coasts. Results show some practices are able to reduce spat mortality with some influencing factors relevant of spat quality (sanitary status, natural or genetic selection) or husbandry practices. Spat “free” of virus is able to survive up to 90% when it is isolated and confined in ponds or nursery, during an epidemic crisis. When outbreaks occurred in oyster beds, natural or nursery spat died in almost same proportions. In 2010, some studies conducted in Mediterranean...
Tipo: Text Palavras-chave: Surmortalité; Naissain; Huître creuse; OsHV-1; Pratiques culturales; Mortality; Spat; Cupped oyster; Crassostrea gigas; OsHV-1; Cultural practices.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00032/14280/25847.pdf
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Facteurs explicatifs de la dynamique spatio-temporelle des mortalités d’huîtres creuses Crassostrea gigas dans la lagune de Thau ArchiMer
Pernet, Fabrice; Lagarde, Franck; Cesmat, Ludovic; Jeannée, Nicolas; Barret, Jean; Le Gall, Patrik; Quere, Claudie; Fiandrino, Annie; Miguet, Marine; Boulet, Hélène; Keck, Nicolas; Roque D'Orbcastel, Emmanuelle.
Cette étude avait pour objectif principal de déterminer les facteurs explicatifs de la dynamique spatio-temporelle des surmortalités d’huîtres creuses dans la lagune de Thau. Les facteurs étudiés étaient : la pathologie (détection d’ADN appartenant à l’Ostreid herpesvirus 1 OsHV-1 et au groupe bactérien Vibrio splendidus), l’état énergétique des huîtres (triglycérides, sucres totaux et acides gras comme marqueurs trophiques), les pratiques d’élevage et l’hydrodynamique. Afin de répondre à ces objectifs, nous avons suivi la mortalité d’un lot d’huîtres sentinelles, âgé de moins d’un an, maintenu indemne de mortalité, dans lequel on ne détectait pas d’ADN appartenant à OsHV-1. Le lot a été déployé mi-mars 2011 sur 106 stations dans la lagune de Thau, où les...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00092/20355/18015.pdf
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Mass mortalities of Pacific oysters Crassostrea gigas reflect infectious diseases and vary with farming practices in the Mediterranean Thau lagoon, France ArchiMer
Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Corporeau, Charlotte; Degremont, Lionel; Lagarde, Franck; Pepin, Jean-francois; Keck, Nicolas.
Experiments were conducted to investigate the effects of farming sites, seeding date, age of oysters, ploidy level and rearing structures on mortality, pathogens and energetic reserves of oysters Crassostrea gigas. Mortality of oysters coincided with infections involving, primarily, the ostreid herpesvirus OsHV-1 and, secondarily, bacteria of the group Vibrio splendidus, when seawater temperature was between 17 and 24°C. Mortality started in early May and lasted until late September, with an interruption in July and August when seawater temperatures rose above 24°C. Mortality varied with farming site: oysters maintained in the open Mediterranean Sea showed no mortality and OsHV-1 was occasionally detected at a low level only, while oysters maintained in...
Tipo: Text Palavras-chave: Pacific oyster; Mortality; Disease; Farming practices; Hydrodynamics; Energetic.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00086/19739/17379.pdf
Registros recuperados: 76
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